Vertebrate Animal Research
Students considering a project that involves vertebrate animals in
ANY way, either directly or indirectly, have a higher level of responsibility and must ensure that they comply with all ISEF rules. Students should download the
ISEF Rules and Guidelines
and carefully review the Vertebrate Animals Rules. Students who are not prepared to take on this responsibility should choose a different area of research.
Important: Terra North Jersey STEM Fair does NOT accept vertebrate animal projects that are subject to ISEF Vertebrate Animal Rules specific to research conducted at a Regulated Research Institution (RRI).
This fair also does NOT accept projects using tissues, organoids, or body fluids obtained from vertebrate animals that were euthanized as part of ANY study performed at an RRI. Review the information below for details about the types of projects that may participate in the Terra North Jersey STEM Fair.
Note: The use of publicly available datasets involving vertebrate animal or tissue studies is exempt from these rules.
TNJSF SRC Vertebrate Animal Rules
Terra North Jersey STEM Fair will accept only vertebrate animal projects that comply with the ISEF Vertebrate Animal Rules for projects conducted at School/Home/Field, regardless of where the work is performed.
Vertebrate Animal Projects as Defined by the TNJSF SRC
A vertebrate animal study is a scientific research project involving the use of vertebrate animals, either directly or indirectly. Under ISEF rules, vertebrate animals include all nonhuman vertebrates, including fish, at hatching or birth; live nonhuman mammalian embryos or fetuses; tadpoles; bird and reptile eggs beginning 72 hours before hatching; zebrafish past 168 hours post-fertilization; and cephalopods.
School/Home/Field Vertebrate Animal Rules
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Research may involve only agricultural, behavioral, observational, or supplemental nutritional studies on animals.
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Research may involve only non-invasive and non-intrusive methods that do not negatively affect an animal’s health or well-being.
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Projects must comply with all applicable ISEF Vertebrate Animal Rules.
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Work performed at a school, home, or field location must be reviewed and approved by the TNJSF SRC before experimentation begins.
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If the work is performed at an RRI, it must be part of an IACUC-approved study that complies with School/Home/Field rules. Prior approval from the TNJSF SRC is recommended but not required.
Projects Conducted at a Regulated Research Institution That Will Not Be Accepted
The TNJSF SRC will NOT accept projects that are subject to ISEF Vertebrate Animal Rules specific to research conducted at an RRI, including, but not limited to:
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Research in which animals experience more than momentary or slight pain or distress that must be relieved using IACUC-approved anesthetics, analgesics, and/or tranquilizers.
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Research involving food or fluid restriction exceeding 18 hours.
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Research involving nutritional deficiency.
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Research involving substances or drugs with unknown effects.
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Research in which animals are euthanized for tissue removal and/or pathological analysis.
Under TNJSF SRC policy, a student who worked on ANY part of an institution’s vertebrate animal study that falls into any of these categories and/or involved euthanized animals is subject to this restriction. This applies regardless of whether the student handled the animals directly and includes projects involving tissue analysis, data collection, and/or data analysis.
TNJSF SRC Vertebrate Tissue and Body Fluids Rules
Terra North Jersey STEM Fair will accept only certain types of studies involving fresh or frozen tissue, blood, or body fluids obtained from humans and/or vertebrates under the ISEF Tissue and Body Fluid Rules. These restrictions also apply to projects involving data collection and/or data analysis of tissue or body fluids from studies performed at an RRI.
Vertebrate Tissue and Body Fluid Projects Allowed by the TNJSF SRC
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Research involving established human and/or mammalian cell lines and tissue culture collections registered with a certified cell bank, such as ATCC, ECACC, or JCRB.
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A project involving a student researcher using their own body fluids, provided the fluids are not cultured.
Rules and Requirements
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Projects must comply with the applicable sections of the ISEF Tissue and Body Fluid Rules.
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Work performed at a school, home, or field location must be reviewed and approved by the TNJSF SRC before experimentation begins.
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If the work is performed at an RRI, it must take place in a laboratory approved by the institution’s IBC for the appropriate biosafety level. Prior approval from the TNJSF SRC is recommended but not required.
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Students must upload all safety training documentation to the Terra entry system for work performed at BSL-1 or BSL-2.
Exempt Vertebrate Studies Allowed by the TNJSF SRC
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Studies involving deceased vertebrate organisms obtained from the field, including necropsy and forensic projects.
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Fresh or frozen meat and meat by-products obtained from food stores, restaurants, or packing houses, as well as eggs or pasteurized milk.
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Hair, hooves, nails, and feathers.
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Teeth that have been sterilized to kill any blood-borne pathogens that may be present.
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Fossilized tissue or archaeological specimens.
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Prepared fixed tissue obtained from a biological supply company.
How to Seek Prior Approval from the TNJSF SRC
Students planning vertebrate animal, tissue, or body fluid research should follow the prior approval process below before beginning their work.
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Submit your entry with all required documentation through the Terra Entry System.
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If your project requires prior approval from the TNJSF SRC before the new software release, email a prior approval request and your Research Plan to the
TNJSF Scientific Review Committee
for feedback and instructions.
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Prior approval submissions must include a ISEF Regulated Research Institutional/Industrial Setting Form (2C)Form 2C with all questions answered, especially those concerning the student’s role in the research.
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The Research Plan must clearly distinguish the work and procedures performed by the mentor from those performed by the student.
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Once your entry is complete in the Terra Entry System, you must request prior approval through the system.
If Prior Approval Is Not Obtained
Students must enter the fair through the normal application process by the applicable Entry Deadline. See the
Student Deadlines
page for details.
The entry must be complete, and an SRC review must be requested through the
Terra Entry System by the applicable deadline.
Warning: If prior approval is not obtained, the student assumes the risk that the TNJSF SRC may disqualify the project after the work has already been completed.